Cell-Cell Interactions: Co-Culture

Growth Factor Induced Proliferation of T47D Cells

Growth Factor Induced Proliferation of T47D Cells

Monitoring of T47D cellular response to H295R estrogen signaling. Estrogen and progesterone receptor positive T47D breast cancer cells were grown on xCELLigence E-plates. After a coculture insert containing H295R adrenal corticocarcinoma cells, known to produce estrogen and progesterone, was placed on the E-plate. The response of the T47D cells to the secreted hormones was suppressed by the application of estrogen synthesis inhibitors (A) Ketoconazole and (B) Anastrazole to the stimulatory H295R cells. (Unpublished data from ACEA Biosciences.)

Key Benefits of Using xCELLigence for Studying Cell-cell Interaction:
  1. Label free environment allows detection of responses due to stimulation of endogenous receptors
  2. Kinetic response profiles may be diagnostic for specific pathways
  3. Real time data provides comprehensive information on cell responses over long time periods
  4. E-Plate Insert offers an easy to setup co-culture platform with minimal handling
xCELLigence Research Grant

The research grant winner will be provided access to the xCELLigence Real Time Cell Analysis (RTCA) DP instrument, consumables, and consultation for up to 6 months.  Apply by December 15, 2019.

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Cell-Cell Interaction Supporting Information:

  • Cell-Cell Interaction – Compatible xCELLigence System:
3×16 wells1×96 wells6×96 wells
  • Cell-Cell Interaction Publications:
  1. A unique co-culture model for fundamental and applied studies of human fetoplacental steroidogenesis and interference by environmental chemicals.  Thibeault AA, Deroy K, Vaillancourt C, Sanderson JT.  Environ Health Perspect. 2014 Apr;122(4):371-7.
  2. Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA).  Roshan Moniri M, Young A, Reinheimer K, Rayat J, Dai LJ, Warnock GL.  Cytotechnology. 2015 Mar;67(2):379-86.