Immune Cell Activation

Real-Time Monitoring of Mast Cell Degranulation in RBL-2H3 Mast Cell Line

(A) Real-time acquisition of impedance-based measurements captures immediate kinetic profi le changes of RBL-2H3 when sensitized with IgE and subsequently stimulated with DNP-BSA. (B) Rhodamine-phalloidin staining of sensitized and activated RBL-2H3 cells performed in parallel to the impedance assay indicates that stimulated cells undergo cytoskeleton rearrangements which correlate with the IgE-mediated impedance responses. (Data and figures adapated from Abassi YA., et. al., 2004).

Real-Time Monitoring of T-cell Activation by CD3 and CD28

Dynamic monitoring of T cell activation using the xCELLigence system. (A) Jurkat cells were seeded onto coated E-plates in the presence of indicated concentrations of anti-CD3 and anti-CD28 functional antibodies, shown as cell index (CI). (B) The dose response curve was plotted using the Cell Index Value at the time point indicated with the dotted line in panel A. (C) Effect of mixed anti-CD3 and anti-CD28 antibodies on rearrangement of actin cytoskeleton. Actin staining is shown in green and nuclear staining in blue. The left panel image shows negative control cells and the right panel image shows cells stimulated with anti-CD3 and anti-CD28 functional antibodies at 1 mg/ml. (Data and figure adapted from Guan N, et. al., 2013).

Key Benefits of Using xCELLigence for Studying Immune Cell Activation:
  • Novel assay platform to support research on allergic inflammation.
  • Sensitive and rapid real-time readout for morphology changes associated with immune cell activation.
  • Screening for modulators of IgE-function and mast cell activation.
  • Eliminate time-consuming, laborious sample processing required by the standard assay.
xCELLigence Research Grant

The research grant winner will be provided access to the xCELLigence Real Time Cell Analysis (RTCA) DP instrument, consumables, and consultation for up to 6 months.  Apply by December 15, 2019.

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Immune Cell Activation Supporting Information:

  • Immune Cell Activation Publications:
  1. Label-free monitoring of T cell activation by the impedance-based xCELLigence system. Guan N, Deng J, Li T, Xu X, Irelan JT, Wang MW. Mol Biosyst. 2013 May;9(5):1035-43.
  2. Label-free, real-time monitoring of IgE-mediated mast cell activation on microelectronic cell sensor arrays. Yama A. Abassi, Jo Ann Jackson, Jenny Zhu, James O’ Connell, Xiaobo Wang, Xiao Xu. Journal of Immunological Methods. 2004;292 (1-2):195-205.