xCELLigence RTCA eSight™

Biosensor Technology meets Live Cell Imaging

The Power of Live Cell Imaging Now Combined with the Sensitivity of xCELLigence Biosensor RTCA Technology

EXCEPTIONAL VERSATILITY: For the first time, you have a system that combines the power of our popular label-free xCELLigence Real Time Cell Analysis technology, with the benefits of live cell imaging in 3 colors: Red, Green, and Blue.

GENERATE PHYSIOLOGICALLY RELEVANT DATA: Easily monitor cell health, strength of adhesion, changes in morphology, proliferation, and cytolysis in primary cell cultures, or with standard tissue culture cell lines. The eSight provides unprecedented insight into cellular mechanisms of action and functionality.

TRULY INFORMATIVE LIVE CELL IMAGING: The eSight provides brightfield capabilities, the flexibility of 3 fluorescence channels, a plethora of well plate formats, and the capability of user-defined schedules. Each cradle can be run and scheduled independently, which makes the system ideal for a multi-user environment.

VERY FAST: You can now read a 96 well plate in ~15 seconds with the xCELLigence biosensor technology, and then gain further insight into your studies with image-based measurements live, simultaneously, and in real time.

Two Complementary Modalities, One Experiment, Easy Workflow

Single Setup For Dual MeasurementsMulti-modal Data AcquisitionInformation Rich & Powerful Analysis
Live cell imaging and real-time biosensor measurements can be performed on the same cell populations, which provides insightful information on cell behavior. Simply place plates in incubator, set up real-time data acquisition & analysis parameters, and walk away.Automatically acquire data with the proprietary xCELLigence biosensor technology and overlay images in real time. The powerful RTCA software integrates two data types into one temporal display.Cell analysis can be displayed and exported into diverse formats, which include RTCA images, KT50 (time to reach 50% cytolysis at a given E:T ratio), % Cytolysis dose response, or IC50 dose response curves.
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Functional Overview

The xCELLigence technology utilizes proprietary microtiter plates (E-Plates) embedded with gold biosensors at the bottom of each well, which serve to non-invasively quantify cell behavior.  Over the course of an experiment, the biosensors monitor cell metrics such as proliferation, adhesion strength, changes in morphology, migration, differentiation, and much more.  Measurements are exceptionally fast and provide exquisite temporal resolution so that all relevant responses can be measured in seconds, minutes, hours, or days. In concert with the biosensor measurements, cell images can be captured in real-time, thereby providing a spatial and temporally dynamic view of cell populations, which can further validate time-dependent cell health and behavior at an unprecedented level of detail for any cell based assay.

The streamlined workflow, high reproducibility, and quantitative kinetics of the eSight system makes it ideal for a wide range of cell-based assays such as proliferation, cytotoxicity, and apoptosis.  The example below illustrates the monitoring of immune cell-mediated killing of cancer target cells in real-time.  MCF7 breast cancer cells were transfected with a lentivirus expressing a red fluorescent protein (eLenti Red, Cat# 8711011), seeded on an E-Plate for 25 hours, and then treated with NK92 cells at different Effector:Target (E:T) ratios.

Figure 1.1 Images taken before (left), or 12hr (center) and 30hr (right) after NK92 effector cells addition at an E:T ratio of 2.5:1 allow visualization of target cell (red) death over time.

Figure 1.2 Effectors addition causes cancer cell death in a E:T ratio-dependent manner as shown by biosensor (left) and imaging (right) measurements. Red fluorescent cell count indicates number of living target cells.

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IMT Handbook

Download xCELLigence eSight CAR T Application Note

Download App Note
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Webinar on Demand: Modeling anti-tumor function of human T cells

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Watch the Introductory Video for the xCELLigence eSight

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Other Resources:


Ordering Information

ProductDESCRIPTIONCatalog Number
xCELLigence RTCA eSight BundlexCELLigence RTCA eSight Instrument
xCELLigence RTCA eSight Control Unit (desktop computer pre-loaded with software) and Monitor
eSight E-Plate VIEW 96 (6 plates)96-well glass plate. Each well contains a biosensor-free viewing window to facilitate imaging00300601020
eSight E-Plate VIEW 96 (36 plates)96-well glass plate. Each well contains a biosensor-free viewing window to facilitate imaging00300601030