xCELLigence RTCA Software Pro

Software Designed for Immunotherapy Applications

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Powerful Immunotherapy Module at Your Fingertips

RTCA Software Pro is an integrated software package for running and analyzing real time cell analysis data from xCELLigence RTCA DP, SP, and MP instruments. This new version has two modules: RTCA Basic and Immunotherapy. The immunotherapy module is designed to measure cytolysis of target tumor cells by immune effector cells and other molecules. Streamline your experimental design and analysis with this new module.



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Ideal for Various Types of Immunotherapy Studies:

  • NK cells
  • CAR-T cells
  • Bi-specific T cell Engagers (BiTEs)
  • Immune checkpoint inhibitors
  • Antibody dependent cell mediated cytotoxicity (ADCC)
  • Tumor-Infiltrating Lymphocytes (TILs)
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Generate Publication Ready Data
Select and simultaneously display relevant data from specific wells. Export % cytolysis time plots or bar graphs at specific time points. With the detailed kinetic curves, determine the amount of time it takes to kill 50% of the target tumor cells (KT50).

Easy Experimental Analysis
Quickly evaluate efficacy of different treatments with automated normalization and compensation features. Measure treatment efficacy against various controls including target cells alone, effector cells alone, target + mock effectors, and full lysis control.

More Accurate & Reproducible Cytolytic Data
Real time effector cytolysis can be consistently calculated at low, physiologically relevant E:T ratios.

Simple Experimental Set Up
Target cells, effectors, and treatments are easily introduced based on the experiment and well content. The interactive software interface automatically recognizes control wells based on plot type selection and normalization.



Easily Visualize Well Content
For each well, target cells, effectors, or treatments can be visualized individually or in combinations through a pie chart, enabling quick comparison between wells.

Expand your analysis capabilities with the Immunotherapy Module
With the Immunotherapy Module, experimental set up and data analysis are made even easier.

Download xCELLigence RTCA Software Pro Brochure

Featured xCELLigence RTCA systems for immune cell killing assays:

Dual PurposeSingle PlateMulti PlateHigh Throughput
3×16 wells1×96 wells6×96 wellsUp to 4×384 wells

Additional Information:

  • Research Presentation Videos:

Jason Maynes Video

Utilization of a Label-Free Real-Time Cell Analysis Technology for Cancer Immunotherapy Applications 

Yama Abassi, Ph.D. (ACEA Biosciences)

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Test Alt

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T-Cells

Dr. Keith Knutson (Mayo Clinic and VGTI)

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Test Alt

Using Impedance-Based Approaches for Measuring Antigen-Specific Cytotoxic T cell Activity 

Dr. Keith Knutson (Mayo Clinic and VGTI)

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