Application Notes

QC Lot Files

NovoCyte® QC Particles Lot File


1. Download the QC particles lot file corresponding to the lot number of the QC particles.
2. Save the QC particles lot file to the “\QC\QC Beads” folder in the NovoExpress® installation directory ( the default installation directory for NovoExpressTM is C:\Program Files (x86)\NovoExpress ).
3. Run NovoCyte® QC test.


Note: Please upgrade NovoExpress to 1.0.2 or above version to use the QC particles lot files.(Right click on the corresponding lot number, choose ” save link as “, and save to local)

Research Presentations

Webinar on Demand: Real-Time High Throughput Viral Cytopathic Assays for Vaccine Development, Neutralizing Antibody Screening, and Anti-Viral Therapy

Real-Time High Throughput Viral Cytopathic Assays for Vaccine Development, Neutralizing Antibody Screening, and Anti-Viral Therapy

Across a broad array of applications, virology assays typically monitor changes in the infected host cell over time.  The traditional methods for quantifying these virus-induced cytopathic effects are often labor intensive and only yield endpoint data.

Using gold-electrode biosensors and combined imaging, the xCELLigence family of instruments continuously and non-invasively monitor virus-induced changes in host cell number, size, attachment strength, and barrier function.  This composite readout is an exceptionally sensitive means of tracking the entire life cycle of a virus and interrogating virus-host interactions.

In this webinar we will demonstrate how, with a simple workflow and minimal hands-on time, xCELLigence assays yield an abundance of quantitative kinetic information that is being used in both industry and academia for:

  • Virus titer determination
  • Quantification of neutralizing antibodies
  • Antiviral therapy development
  • Evaluating oncolytic virus efficacy
  • Assessing virus quality/potency/fitness
Watch on Demand


Presented by:

Brandon Lamarche, Ph.D.
Principal Scientist, Head of xCELLigence Application Development
Agilent Technologies, Cell Analysis Division


For Research Use Only. Not for use in diagnostic procedures

Live Technical Webinar: Compensation in Flow Cytometry

Date: June 28, 2018

About this Webinar

Compensation in flow cytometry can often be challenging and sometimes confusing, however doing compensation correctly is absolutely essential for valid and optimal analyses of multicolor flow cytometry experiments. This webinar will discuss fluorescence spillover, compensation, how to calculate compensation accurately, best practices and considerations for control samples, and some challenges that may be encountered. Participants will learn tips and tricks on how to prepare high-quality compensation controls and how to create an optimal compensation matrix, resulting in higher confidence in data analyses and more robust data.

About the Presenter

Heather Paich, Ph.D.
Field Application Scientist
ACEA Biosciences Inc.

Heather Paich received her PhD in Nutritional Biochemistry at the University of North Carolina at Chapel Hill where her research focused on how obesity affects the immune response to pandemic H1N1 influenza. After completing a Fogarty Global Health Post-Doctoral Fellowship in Cape Town, South Africa, where her work focused on infectious diseases, she joined ACEA Biosciences, where she is currently a Senior Field Application Scientist for Flow Cytometry.

A Flow Cytometry Method for Detecting Bacteria in Water

About this Webinar

Regulatory testing for microbiological water quality is often conducted by growing microbes on agar medium in visible colonies. However, this is a time-consuming process and not all bacteria can grow in media and therefore be detected using this method. Flow cytometers with a high sensitivity of detection provide tools for detecting and analysing microbes independent of their cultivability and allows precise and rapid determinations of microbial bulk parameters and delivers detailed information on the general microbial state.

During this webinar, we will cover:

1. Advantages of flow cytometry for microbiological water quality testing
2. How to determine absolute counts, nucleic acid content, activity, and classification of bacteria
3. Tips on running a flow cytometry experiment for microbial analysis
4. Data analysis of treated water samples using the NovoExpress software


About the Presenter

Stephanie Ting, M. Eng
Field Application Scientist – SE Asia, ANZ, HK&Macau, India,
ACEA Biosciences Inc.

Stephanie received her Masters in Mechanical Engineering from the University of Tokyo, Japan where she studied the effects of mechanobiological stimuli on the production of cartilage-like tissues using mesenchymal stem cells. Currently, she is the Field Application Scientist for SE Asia, ANZ, HK&Macau, and India.


WEBINAR – Cell Cycle Analysis by Flow Cytometry

About this Webinar

DNA content analysis using flow cytometry is a powerful tool that can determine the effect of treatments on cell cycle and ploidy in particular relevance to tumours. However, how do you determine which DNA-binding dye to use? How do you accurately differentiate the different phases in the cell cycle in your population of cells? This webinar will teach the methods for cell cycle analysis with the following topics:

During this webinar, we will cover:

  • Phases of a typical mammalian cell cycle
  • Characteristics of Nucleic Acid Stains
  • How to choose the right fluorochromes and properly prepare your cells
  • DNA ploidy & tumours

Session One:

Date: Thursday, December 21, 2017
Time: 8 AM SINGAPORE | 9 AM TOKYO | 4 PM PST (Dec. 20)

Session Two:

Date: Thursday, December 21, 2017
Time: 7 AM PST | 10 AM EST | 4 PM PARIS


About the Presenter

Stephanie Ting, M. Eng
Field Application Scientist – SE Asia, ANZ, HK&Macau, India,
ACEA Biosciences Inc.

Stephanie received her Masters in Mechanical Engineering from the University of Tokyo, Japan where she studied the effects of mechanobiological stimuli on the production of cartilage-like tissues using mesenchymal stem cells. Currently, she is the Field Application Scientist for SE Asia, ANZ, HK&Macau, and India.



WEBINAR – Measuring Calcium Flux by Flow Cytometry

Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. Flow cytometry allows real time measurement of a calcium flux response utilizing fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. In this webinar we will discuss the basics of calcium flux measurements by flow cytometry, providing specific details on experiment setup, acquisition, and analysis on the NovoCyte flow cytometer.

Key topics to be covered:
1. Essentials of calcium measurements by flow cytometer
2. Calcium flux experimental setup and acquisition on the NovoCyte
3. Detailed analysis of calcium flux in a T cell line using calcium indicator dye Fluo-4/AM

Learn More about the NovoCyte Flow Cytometer       Click here


Lauren Jachimowicz, Ph.D. 
Application Development Scientist, ACEA Biosciences, Inc.
Lauren Jachimowicz received her PhD in Biological Science from the University of California, San Diego where she studied the role of the circadian clock genes in T cells. Currently, she is the Application Development Scientist for flow cytometry at ACEA Biosciences advancing flow cytometry into new fields of research.

WEBINAR: Flow Cytometric Assessment, Quantification and Regulation of Human Neutrophil Extracellular Traps (NETS)

At the CYTO 2017 meeting Dr. Adriano Rossi and Dr. Calum Robb from the University of Edinburgh described the development of a new flow cytometry-based assay for studying NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms. Using the NovoCyte® flow cytometer, they demonstrate the detection and quantification of neutrophil extracellular traps (NETs), as well as analysis of the regulatory mechanisms involved.

WEBINAR: Flow Cytometry: Simplifying Assay Panel Design and WorkFLOW

About this Webinar

Researchers and flow cytometry users spend a significant amount of time designing flow assay panels and analyzing their samples.

In this webinar, viewers will have the chance to learn about  tools and recent developments in flow cytometry  that simplify assay design and workflow. Our speakers will explore the capabilities of benchtop and cost-effective flow cytometry systems and the easiest way to design flow panels.

Webinar Features

  • Design and capabilities of a NovoCyte flow cytometer
  • New features that simplify setup, acquisition, and analysis
  • How to design multicolor fluorescent panels using FluoroFinder’s experiment design platform
  • Tips for building better panels in less time

Featured Speakers:

Garret Guenther, Ph.D.
ACEA Biosciences.

Jeff Jensen, Ph.D.
FluoroFinder, LLC


WEBINAR – Flow Cytometry: Emerging Instrumentation and Application Trends | Wed, Feb 15, 2017

Flow cytometry remains an essential tool for cell analysis. Although often relegated to specialized core facilities, flow cytometry instruments are in fact becoming increasingly commonplace in life science labs.

In this special webinar, viewers will have the chance to learn about recent developments in flow cytometry technology, including the emergence of lower cost, smaller flow cytometry options with the capability of analyzing multiple fluorescent colors. Our speakers will explore the design, development, and uses for smaller, more cost-effective flow cytometry systems in research labs. In this webinar, you will gain insights on:

  • Design and development of the latest multicolor flow cytometry instruments
  • Application of flow cytometry techniques for different experimental needs
  • How analyzing multiple cellular parameters using different  fluorescent colors can extend experimental possibilities
  • How to effectively analyze and interpret your flow cytometry data sets
Learn More about the NovoCyte Flow Cytometer       Click here


Paul K. Wallace, PhD
Roswell Park Cancer Institute
Paul Wallace has served since 2003 as Director of the Flow and Image Cytometry Department at the Roswell Park Cancer Institute (RPCI) in Buffalo, NY, where he is a Professor of Oncology. He is also an Associate Professor of Biotechnical and Clinical Laboratory Sciences at the University at Buffalo. His primary expertise and board certification is in the flow cytometric diagnosis of hematological malignancies.

Christopher Groves 
MedImmune, Inc. 
Christopher Groves is a Senior Manager at MedImmune Inc., where he heads a keystone technology group  challenged with expanding knowledge of disease mechanisms while also delivering innovative solutions in drug discovery and manufacturing that provide scientists access to cutting-edge technological capability.

William Telford, PhD 
Experimental Transplantation and Immunology Branch, NCI-NIH 
William Telford is the Head of the NCI-NIH Flow Cytometry core facility, which provides state-of-the-art flow cytometry as well as imaging technology and instrumentation to users in the research community.


Patrick C.H. Lo, PhD 
Senior Editor, BioTechniques


Bench Tip Video: Simplifying Cell Proliferation Studies with Flow Cytometry



xCELLigence – A Transformative Technology in Cancer Research (2/2)

xCELLigence – A Transformative Technology in Cancer Research (1/2)

Checkpoint Inhibitor Research Challenges

Targeting Checkpoint Inhibitors for Cancer Treatment: The Possibilities